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Evaluation of the aqueous compatibility of the C-NAF derivatives in <t>DMEM.</t> UV-vis absorption spectra of (A) C-NAF, (B) C-NAF-M, and (C) C-NAF-B7 recorded at increasing concentrations in <t>Dulbecco's</t> Modified Eagle Medium (DMEM) containing less than 1% (v/v) DMSO. Insets show the corresponding Beer–Lambert calibration plots obtained by monitoring the absorbance at 534 nm. The R 2 values, and molar absorption coefficients ( ε ) are indicated in each inset.
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Evaluation of the aqueous compatibility of the C-NAF derivatives in <t>DMEM.</t> UV-vis absorption spectra of (A) C-NAF, (B) C-NAF-M, and (C) C-NAF-B7 recorded at increasing concentrations in <t>Dulbecco's</t> Modified Eagle Medium (DMEM) containing less than 1% (v/v) DMSO. Insets show the corresponding Beer–Lambert calibration plots obtained by monitoring the absorbance at 534 nm. The R 2 values, and molar absorption coefficients ( ε ) are indicated in each inset.
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Evaluation of the aqueous compatibility of the C-NAF derivatives in <t>DMEM.</t> UV-vis absorption spectra of (A) C-NAF, (B) C-NAF-M, and (C) C-NAF-B7 recorded at increasing concentrations in <t>Dulbecco's</t> Modified Eagle Medium (DMEM) containing less than 1% (v/v) DMSO. Insets show the corresponding Beer–Lambert calibration plots obtained by monitoring the absorbance at 534 nm. The R 2 values, and molar absorption coefficients ( ε ) are indicated in each inset.
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Evaluation of the aqueous compatibility of the C-NAF derivatives in <t>DMEM.</t> UV-vis absorption spectra of (A) C-NAF, (B) C-NAF-M, and (C) C-NAF-B7 recorded at increasing concentrations in <t>Dulbecco's</t> Modified Eagle Medium (DMEM) containing less than 1% (v/v) DMSO. Insets show the corresponding Beer–Lambert calibration plots obtained by monitoring the absorbance at 534 nm. The R 2 values, and molar absorption coefficients ( ε ) are indicated in each inset.
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Evaluation of the aqueous compatibility of the C-NAF derivatives in <t>DMEM.</t> UV-vis absorption spectra of (A) C-NAF, (B) C-NAF-M, and (C) C-NAF-B7 recorded at increasing concentrations in <t>Dulbecco's</t> Modified Eagle Medium (DMEM) containing less than 1% (v/v) DMSO. Insets show the corresponding Beer–Lambert calibration plots obtained by monitoring the absorbance at 534 nm. The R 2 values, and molar absorption coefficients ( ε ) are indicated in each inset.
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Evaluation of the aqueous compatibility of the C-NAF derivatives in DMEM. UV-vis absorption spectra of (A) C-NAF, (B) C-NAF-M, and (C) C-NAF-B7 recorded at increasing concentrations in Dulbecco's Modified Eagle Medium (DMEM) containing less than 1% (v/v) DMSO. Insets show the corresponding Beer–Lambert calibration plots obtained by monitoring the absorbance at 534 nm. The R 2 values, and molar absorption coefficients ( ε ) are indicated in each inset.

Journal: RSC Advances

Article Title: Design and synthesis of hydrolyzable coumarin–naphthalimide dual probes for NIR fluorescence detection and selective photodynamic activity in cancer cells

doi: 10.1039/d6ra08240h

Figure Lengend Snippet: Evaluation of the aqueous compatibility of the C-NAF derivatives in DMEM. UV-vis absorption spectra of (A) C-NAF, (B) C-NAF-M, and (C) C-NAF-B7 recorded at increasing concentrations in Dulbecco's Modified Eagle Medium (DMEM) containing less than 1% (v/v) DMSO. Insets show the corresponding Beer–Lambert calibration plots obtained by monitoring the absorbance at 534 nm. The R 2 values, and molar absorption coefficients ( ε ) are indicated in each inset.

Article Snippet: Human embryonic kidney cells HEK-293 (ATCC CRL-1573), used as a healthy model, and MCF-7 adenocarcinoma breast cancer cells (ATCC HTB-22) were cultured in Dulbecco's Modified Eagle's Medium (DMEM) supplemented with 10% fetal bovine serum (FBS), 100 U mL −1 penicillin, and 100 μg mL −1 streptomycin at 37 °C, 5% CO 2 , and 100% humidity.

Techniques: Modification